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Figure 10.

Spectrograms of adult and embryonic RPCH-activated motor patterns. , Adult PD, LP and DG spectrograms, total recording time in 10 m RPCH from Figure 9 . , Embryonic pdm, lpm, and dgm spectrograms, total recording time in 10 m RPCH, preparation shown in Figure 9 .

When we calculated the mean fundamental frequencies of activity in adults, we found that LP neurons were silent in control saline (data not shown), but were active in RPCH at 0.17 ± 0.09 Hz ( n = 7) ( Clearance Cheapest Price Authentic Cheap Online Opening Ceremony Ryder Embellished Ankle Boots Buy Cheap Footaction Sale 2018 Unisex Safe Payment OEV164QId
A ). RPCH did not significantly change the mean fundamental frequency of activity in adult PD neurons compared with control saline (mean fundamental frequencies: in control saline, 0.13 ± 0.05 Hz, n = 7; in RPCH, 0.19 ± 0.11 Hz, n = 7; paired t test, p = 0.17, n = 7) ( Clearance Authentic Prada Leather SquareToe Ankle Boots From China Online Free Shipping Very Cheap Sale For Cheap Cheap Excellent NEIBgz11
A , control saline data not shown). In RPCH, DG neurons produced bursts at a mean fundamental frequency (0.04 ± 0.01 Hz, n = 7) that was significantly slower than that of DG neurons in control saline (0.83 ± 0.53 Hz, n = 7; paired t test, p = 0.01, n = 7) ( Tbar sandals cafe/gold Shopping Online Purchase Cheap Price 0xsqlF6v
A , control data not shown). In RPCH, the mean fundamental frequencies of activity in LP and PD were not significantly different (one-way ANOVA, p = 0.64, n = 7), but the mean frequencies of activity in both LP and PD neurons were faster than DG neurons (one-way ANOVA, p = 0.004, n = 7) ( Fig. 11 A ).

Figure 11.

Summary of frequency and phase relationships of adult and embryonic RPCH-activated motor patterns. , Output from adult ( , ) and embryonic ( , ) neurons during 10 m RPCH application. , The mean fundamental frequencies of activity in adult PD and LP neurons were not significantly different (one-way ANOVA, = 0.64, = 7), but both were faster than the mean fundamental frequency of activity in adult DG neurons (one-way ANOVA, = 0.004, = 7). Significant difference, ** < 0.01. , The mean fundamental frequencies of activity in embryonic lpm and pdm were not statistically different (one-way ANOVA, = 0.80, = 7), but both were both faster than the mean fundamental frequency of activity in embryonic dgm (one-way ANOVA, = 0.001, = 7). , Phase relationships of activity in adult LP ( = 7) and DG ( = 7) neurons in the PD phase. , Phase relationships of activity in embryonic lpm ( = 7) and dgm ( = 7) neurons in the pdm phase.

In control saline, all embryonic neurons were silent after neuromodulatory inputs were removed. In RPCH, the mean fundamental frequencies of the activity in lpm (0.10 ± 0.03 Hz, n = 7) and pdm (0.11 ± 0.04 Hz, n = 7) were not statistically different (one-way ANOVA, p = 0.80, n = 7), and the mean fundamental frequencies of activity in both lpm and pdm were faster than dgm (0.04 ± 0.14 Hz, n = 7) (one-way ANOVA, p = 0.001, n = 7) ( Fig. 11 B ). RPCH-induced events were typical of EJPs.